Table 1.
Summary of animals included in this study
| Animal number | Conditioning | HCT | VCA timing1 | Stable mixed chimerism2 | VCA survival (days)3 | Skin graft survival4
|
Additional comments | |
|---|---|---|---|---|---|---|---|---|
| Donor | Third party | |||||||
| 17468 | Yes | Yes | Delayed | Yes | >46 | – | – | Central line complications, euthanasia day 46 |
| 17469 | Yes | Yes | Delayed | Yes | >400 | >275 | – | – |
| 20311 | Yes | Yes | Delayed | Yes | >504 | >338 | 24/24 | Cutaneous GvHD stage 1 diagnosed day 50. CyA days 76–106. Complete resolution, no recurrence |
| 20313 | Yes | Yes | Delayed | Yes | >115 | – | – | Cutaneous GvHD stage 1 day 45, progressive to stage 3 by day 70. Relapsing-remitting course treated with CyA days 86–110, Methylprednisolone days 71–114, 130–150, 160–179 |
| 20312 | Yes | No | Delayed | No | 9 | – | – | – |
| 20680 | Yes | Yes | Simultaneous | Yes | >486 | >335 | 42/56 | – |
| 20681 | Yes | Yes | Simultaneous | Yes | >139 | – | – | – |
| 20989 | Yes | No | Simultaneous | Yes | 79 | – | – | – |
| 17519 | No | No | N/A | No | 6 | – | – | – |
| 17520 | No | No | N/A | No | 6 | – | – | – |
CyA, cyclosporine A; GvHD, graft versus host disease; HCT, hematopoietic cell transplantation; VCA, vascularized composite allograft.
1
VCA transplantation was either delayed (85–150 days post-HCT) or simultaneous to induction of mixed chimerism (within 56 h of first infusion of donor hematopoietic cells). Control animals 17519 and 17520 received VCA with neither conditioning nor HCT.
2
Chimerism levels for individual chimeric animals are shown in Figure 2.
3
>denotes rejection-free VCA survival at termination of experiment.
4
>denotes rejection-free skin graft survival at termination of experiment. Animals 20311 and 20680 each received two third-party grafts, survival for each is presented.