Figure 4.
Effects of rosiglitazone on Akt phosphorylation in shLuc and shPTEN cells. Cells were treated with 25 μM rosiglitazone prior to incubation with 5 μg/mL LPS for 15 min. Cell lysates were harvested for determining the levels of phospho-Akt, Akt, and β-actin by Western blotting. Data shown are representative of three individual experiments. Data are expressed as mean ± SEM obtained from three individual cultures. *P< 0.05 compared with the control, untreated group. # P < 0.05 compared with the LPS group.