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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Genet Genomics. 2012 Jan 21;39(2):69–80. doi: 10.1016/j.jgg.2012.01.005

Fig. 6. Diagram shows the gene trap integration in myomesin-3 gene and PCR strategy for analyzing the expression of myomesin-3 and myomesin-3-RFP mRNA transcripts.

Fig. 6

A:. The RFP gene trap is integrated at the intron 24 of myomesin-3. A cDNA fragment covering the junction site of myomesin-3 and RFP fusion was amplified by RT-PCR. Additional sequence of 39 bp from intron 24 and 32 bp from the vector sequence upstream of RFP was found in the myomesin-3-RFP fusion transcript. myomesin-3 GenBank accession No. XM_001921030. B: RT-PCR results show the expression of myomesin-3 mRNA transcripts or myomesin-3-RFP fusion products in wild type or homozygous myom3(mnGt0067) fish embryos.