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. 2014 Feb 25;6(3):591–605. doi: 10.1093/gbe/evu041

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© The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3.— — Saturation plot of the first plus second codon positions (P12) of the 13 mitochondrial protein-genes, drawn for all congeneric and intraspecies ascidian pairs. Continuous violet arrowhead lines: gap between intraspecies and all congeneric distances. Dotted violet arrowhead lines: gap between intraspecies and congeneric distances excluding the cryptic Ciona intestinalis and the Botrylloides leachii–Botrylloides nigrum species pairs. The “x = y” line represents the situation where the number of inferred substitutions is equal to the number of observed differences.