Table 4.
Analysis of metabolite-associated microbial communities
| Metabolite mass and retention time | Putative ID | Colonic region | Correlated OTUs, number ( q <0.2) | Total branch length | Association type | Average positive correlation | Average negative correlation | Number of correlations >0.2 | Number of correlations <−0.2 |
|---|---|---|---|---|---|---|---|---|---|
| Magenta module |
Multiple |
Cecum |
120 |
16.58 |
All positive |
0.16*** |
−0.14*** |
2182*** |
1930*** |
| Green yellow module |
Multiple |
Cecum |
94 |
16.48 |
Mostly positive |
0.14*** |
−0.14*** |
1170*** |
1126*** |
| 342.2631 m/z RT = 4.2272 |
trans-2-Dodecenoylcarnitine |
Cecum |
45 |
11.21 |
Mostly negative |
0.16*** |
−0.14** |
290** |
298*** |
| Pink module |
Multiple |
Cecum |
31 |
9.17 |
Mostly negative |
0.13 |
−0.11 |
90 |
58 |
| Brown module |
Multiple |
Cecum |
23 |
6.81 |
All positive |
0.18** |
−0.12 |
88** |
42 |
| 386.2897 m/z RT = 4.3619 |
3-Hydroxy-cis-5-tetradecenoylcarnitine |
Cecum |
19 |
6.18 |
Mostly negative |
0.13 |
−0.1 |
28 |
20 |
| 230.1845 m/z RT = 0.4078 |
N1,N8-diacetylspermidine |
Cecum |
17 |
6 |
All negative |
0.32*** |
−0.29*** |
102*** |
84*** |
| 596.3475 m/z RT = 3.04 |
? |
Sigmoid |
61 |
8.97 |
All positive |
0.14** |
−0.14*** |
442** |
406** |
| 595.3469 m/z RT = 3.0469 |
L-Urobilin |
Sigmoid |
58 |
10.5 |
All positive |
0.13 |
−0.12* |
326 |
322* |
| 593.3309 m/z RT = 3.0247 |
I-Urobilinogen |
Sigmoid |
54 |
9 |
Mostly positive |
0.15*** |
−0.14*** |
384** |
344** |
| 613.3011 m/z RT = 3.0293 |
Harderoporphyrinogen |
Sigmoid |
52 |
9.02 |
All positive |
0.15*** |
−0.14*** |
376*** |
356*** |
| 605.3348 m/z RT = 3.3185 |
(23S)-23,25-dihdroxy-24-oxovitamine D3 23-(beta-glucuronide) |
Sigmoid |
43 |
10.1 |
Mostly positive |
0.15* |
−0.13* |
222* |
194* |
| 591.3203 m/z RT = 3.0304 |
Mesobilirubinogen |
Sigmoid |
37 |
9.03 |
Mostly positive |
0.13 |
−0.12* |
152* |
122 |
| Salmon module |
Multiple |
Sigmoid |
37 |
8 |
Mostly positive |
0.15* |
−0.14** |
184** |
160** |
| 100.0761 m/z RT = 0.3093 |
2-Hydroxy-2-methylbutanenitrile |
Sigmoid |
32 |
8.37 |
22% Negative, 78% positive |
0.21*** |
−0.16*** |
178** |
196*** |
| 673.3209 m/z RT = 3.3132 |
? |
Sigmoid |
30 |
7.93 |
Mostly positive |
0.15* |
−0.14* |
128** |
110** |
| 434.1867 m/z RT = 0.6621 |
? |
Sigmoid |
26 |
6.54 |
30% Positive, 70% negative |
0.28*** |
−0.21*** |
160*** |
162*** |
| 619.3474 m/z RT = 3.6896 |
? |
Sigmoid |
25 |
9.13 |
Mostly positive |
0.13 |
−0.13* |
76* |
48 |
| 124.0395 m/z RT = 0.3735 |
Nicotinate |
Sigmoid |
21 |
7.47 |
Mostly negative |
0.18** |
−0.11 |
58* |
30 |
| 615.3154 m/zRT = 3.0227 |
Mesobilirubinogen |
Sigmoid |
18 |
6.31 |
All positive |
0.1 |
−0.08 |
6 |
2 |
| Midnight blue module | Multiple | Sigmoid | 18 | 6 | All positive | 0.1 | −0.08 | 14 | 12 |
Significance values: ***P ≤0.001, **P ≤0.01, *P ≤0.05.
Total branch length is the sum of all branch lengths from Phylogenetic (Newick) trees containing the corresponding operational taxonomic units (OTUs). Retention time (RT) is provided in minutes.