Table 3.
Diversity and count estimates of 16S genes in pyrosequencing libraries after sequence trimming
| Sample | Number of raw 16S rRNA fragment reads | Number of OTUs after pre-cluster analyses b | Shannon entropy | Shannon evenness |
|---|---|---|---|---|
| Biofilm batch 1 |
6011 |
132 |
3.7436 |
0.7620 |
| Biofilm batch 2 well 1a |
5731 |
107 |
3.6971 |
0.7960 |
| Biofilm batch 2 well 2a |
8538 |
158 |
4.1868 |
0.8200 |
| Biofilm batch 3 well 1 |
4835 |
154 |
4.1622 |
0.8284 |
| Biofilm batch 3 well 2 |
5166 |
146 |
4.1082 |
0.8289 |
| Biofilm batch 3 well 3 |
4778 |
148 |
4.2334 |
0.8683 |
| Saliva 1 |
6956 |
208 |
4.7209 |
0.8544 |
| Saliva 2 |
6885 |
243 |
4.7209 |
0.8544 |
| Saliva 3 | 3799 | 179 | 4.5234 | 0.8828 |
Biofilm batches 1, 2 and 3 correspond to replicate biofilm samples grown at different time points. The biofilm batch-2 library was generated from stable isotope probing experiments (see Methods). aWells 1, 2 and 3 correspond to replicate technical samples collected from different sample wells. bNumbers of operational taxonomical units (OTUs) after precluster-analyses using the SILVA reference alignment and the MOTHUR software.