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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Free Radic Res. 2012 Jun 8;46(9):1115–1122. doi: 10.3109/10715762.2012.692785

Figure 5.

Figure 5

Kinetics of the EPR signal intensity decay of 50 μM air-saturated aqueous solutions of HIMD1 (A) and HIMD2 (B) in 50 mM phosphate buffer, pH 7.0, at room temperature, in the presence of reducing agent, ascorbate, 10mM (○); radical initiator AIPH, 80 mM (□); and both, 10 mM ascorbate and 80 mM AIPH (●). The rates of the HIMD1 and HIMD2 decay in the presence of AIPH are about of 65 nM/s and 90 nM/s, respectively. The rate of ROO• generation by AIPH was estimated to be close to 90 nM/s (AIPH decomposition rate constant, kd ≈1.2×10−6 s−1 at 22 °C[53], was approximated from the decomposition rate constants in the temperature range from 40 to 60 °C provided by Waco Pure Chemical Industries, Ltd.; efficiency of the radical escape from the initial radical pair, 0.47[33, 54]).