Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 31;204(7):1141–1156. doi: 10.1083/jcb.201310090

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Kasprowicz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Stimulus-dependent Chc and α-Ada recruitments are blocked upon Dynamin photoinactivation. (A–H) Superresolution imaging of HA-Chc fusion proteins with anti-HA antibodies, using structured illumination microscopy in not stimulated (−KCl) and stimulated (+KCl; 90 mM for 5 min) preparations. (A and B) Labeling of yw; HA-chc controls (yw) and shi^12-12B/Y; HA-chc/shi-4C without FALI at rest (−KCl). Note the presence of Chc in the bouton center and at the bouton periphery as quantified in F (n = 10 boutons from three larvae); see Materials and methods and also Fig. S3. rel., relative. (C–E) Labeling of yw; HA-chc (yw) and shi^12-12B/Y; HA-chc/shi-4C, stimulated with KCl without Dynamin inactivation (blue; C and D) and with Dynamin inactivation using FALI (E). Note that in the stimulated controls (C and D), Chc becomes more concentrated in the bouton periphery than in animals in which Dynamin was inactivated (E) as quantified in G and H (n = 11–12 boutons from four to five larvae); see Materials and methods and also Fig. S3. (I–P) Superresolution imaging of α-Ada using structured illumination microscopy in not stimulated (−KCl) and stimulated (+KCl; 90 mM for 5 min) preparations. (I and J) Labeling of yw controls (yw) and shi^12-12B/Y; shi-4C/+ without FALI at rest (−KCl). Note the presence of α-Ada in the bouton center and at the bouton periphery as quantified in N (n = 9–10 boutons from three to four larvae); see Materials and methods and also Fig. S3. (K–M) Labeling of yw (yw) and shi^12-12B/Y; shi-4C/+, stimulated with KCl without Dynamin inactivation (blue; K and L) and with Dynamin inactivation using FALI (M). Note that in the stimulated controls (K and L), α-Ada becomes more concentrated in the bouton periphery than in animals in which Dynamin was inactivated (M) as quantified in O and P (n = 9–10 boutons from four to five larvae); see Materials and methods and also Fig. S3. SEM is shown in the lighter shade. Arrows, plasma membrane. Bars, 2 µm.