Table 5.

Primer sequences for mutational analysis of EGFR.

Gene	Exon	Primer sequence	Annealing temperature
EGFR (sense)	18	5′-GTG TCC TGG CAC CCA AGC-3′	60
EGFR (anti-sense)	18	5′-CCC CAC CAG ACC ATG AGA-3′	60
EGFR (sense)	19	5′-CAG CAT GTG GCA CCA TCT C-3′	62
EGFR (anti-sense)	19	5′-CAG AGC AGC TGC CAG ACA T-3′	62
EGFR (sense)	20	5′-CAT TCA TGC GTC TTC ACC TG-3′	60
EGFR (anti-sense)	20	5′-CAT ATC CCC ATG GCA AAC TC-3′	60
EGFR (sense)	21	5′-AGC CAT AAG TCC TCG ACG TG-3′	60
EGFR (anti-sense)	21	5′-ACC CAG AAT GTC TGG AGA GC-3′	60