Table 1. The PCR Mixture .

PCR	Volume, tub, µL	Final Concentration
10 X PCR buffer	5	1X
50 mM mgcl 2	1.5	1.5 mM
10mM dNTPS	1	200 µM
10mM PF&PR	2.5	0.5 µM
5 unit / µl polymerase	0.5	2.5 unit
Distilled water	32	-
Template DNA	5	-