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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Science. 2012 Apr 13;336(6078):237–240. doi: 10.1126/science.1215691

Figure 3.

Figure 3

mRNA deadenylation is not required for miRNA-mediated translational repression. (A-D) Length of poly(A) tail was determined using G/I tailing PCR based amplification (Materials and Methods). Positions of over-amplified products with or without (C) poly(A) tail are indicated. GAPDH mRNA poly(A) tail length is shown as a control. M lane represents 100 bp markers. (E) Time resolved progression of miRNA-mediated gene silencing for histone H3 constructs. Normalized levels of protein and mRNA amounts for miRNA-targeted (T) and non-targeted (NT) constructs are shown. Each data point represents an average value from three independent experiments.