HEK-293 cells were transfected with Flag-tagged WT LRRK2, LRRK2 2019S or a kinase dead form of LRRK2 and various epitope-tagged VDAC1, uMtCK, different isoforms of ANTs and VAMP2, either alone or in combination as indicated. Thirty-six hours later, aliquots of cell lysates were saved for the analysis of expressed tagged proteins. The remaining portions of the lysates were immunoprecipitated with an anti-Flag antibody. The immunocomplexes were analysed with anti-HA (A, B and F) and anti-Myc (C, D and E) antibodies.