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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: Cell Metab. 2014 Feb 4;19(2):272–284. doi: 10.1016/j.cmet.2013.12.001

Figure 2. The effect of BAD phosphorylation on gluconeogenesis.

Figure 2

(A) Immunoblot analysis and quantification of relative BAD phosphorylation on S155, S136 and S112 in liver samples derived from C57BL/6J mice fasted overnight and re-fed for 6 hr after overnight fasting.

(B) PTT in Bad +/+ and S155A knock-in mice (n=10–11).

(C) PTT in Bad +/+ and −/− mice following hepatic reconstitution with the indicated adenoviruses (n=16–24). Asterisks in (C) compare Bad −/−: BAD S155D vs. Bad −/−: GFP and the symbols # compare Bad −/−: BAD WT vs. Bad −/−: GFP.

(D) Glucose production in primary Bad −/− hepatocytes reconstituted with the indicated adenoviruses and treated with lactate/pyruvate (n=7–12).

Error bars, ± SEM. *p < 0.05; **p < 0.01, ***p < 0.001; n.s., non-significant.

See also Figure S2.