Figure 3.

Experimental strategies for targeting SB transposition. The common components of the targeting systems include a transposable element that contains the terminal inverted repeats (TIRs, black arrowheads) of the transposon and a GOI equipped with a suitable promoter. The transposase (purple oval) binds to the TIRs and catalyzes transposition. A DNA-binding protein domain (yellow sphere) recognizes a specific sequence (blue box) in the target DNA (parallel lines). A: Targeting with transposase fusion proteins. Targeting is achieved by fusing a specific DNA-binding protein domain to the transposase. B: Targeting with fusion proteins that bind the transposon DNA. Targeting is achieved by fusing a target-specific DBD to another DBD (red sphere) that binds to a specific DNA sequence within the transposable element (red box). In this strategy, the transposase is not modified. C: Targeting with fusion proteins that interact with the transposase. Targeting is achieved by fusing a target-specific DBD to a protein binding domain (PBD, green sphere) that interacts with the transposase. In this strategy, neither the transposase nor the transposon is modified.