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(a) The continuum absorbance changes of wild type at pH 7 (solid lines) and at pH 5 (dotted lines). The first spectra (d0) are shown in cyan, the second spectra (d1) in brown, the third spectra (d2) in red, and fourth spectra (d3) in blue. (b) The difference between the spectra at pH 7 and pH 5 of d1 (brown), d2 (red), and d3 (blue). One division of the ordinate corresponds to 0.00005 and 0.00002 absorbance unit for bacteriorhodopsin at pH 7 in (a) and (b), respectively.
