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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Cereb Blood Flow Metab. 2008 Apr 16;28(7):1361–1368. doi: 10.1038/jcbfm.2008.32

Figure 5.

Figure 5

Effect of neural progenitor cells treated with rhEPO on expression of VEGFR2 in MBECs. Real-time RT-PCR (A) shows VEGFR2 mRNA levels in MBECs cultured with the supernatant harvested from neural progenitor cells treated with rhEPO (EPO), rhEPO and LY294002 (+ LY), or rhEPO and U0126 (+ U). Western blot (B) analysis shows VEGFR2 protein levels in MBECs cocultured with neural progenitor cells treated with rhEPO (EPO), rhEPO and LY294002 (+ LY), or rhEPO and U0126 (+ U). Panel C is quantitative data of VEGFR2 protein levels. Glyceraldehyde-3-phosphate dehydrogenase and actin were used as internal controls for real-time RT-PCR and western blot analysis, respectively. *P < 0.05 and #P < 0.05 versus the control and rhEPO groups, respectively. n = 3/group.