Table 1. Dissociation Constants for Binding of MecI or BlaI to the mec or bla Operatora.
dsDNA | Kd1 (μM) | Kd2 (μM) | Kd3 (μM) | |
---|---|---|---|---|
MecI | mec | <0.01 | 0.67 ± 0.02 | <0.03 |
bla | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.2 | |
BlaI | mec | 0.013 ± 0.006 | 2.8 ± 0.1 | <0.04 |
blab | 0.45 ± 0.07 | 2.6 ± 0.1 | 0.72 ± 0.07 |
Kd1 and Kd2 define dissociation of the monomer from the monomer–DNA and dimer–DNA complexes, respectively, and Kd3 is for dissociation of the dimer from the dimer–DNA complex.
Data from ref (12).