Table 1.
Antibodies used for analysis of immune cell subsets in adipose tissue
| FSC/SSC gate |
Cell population |
Cell subpopulation |
Antibody | Mouse Clone |
|---|---|---|---|---|
| Lymphocyte | ||||
| T cell | CD3 | 145-2C11 | ||
| CD4 + T cell | CD4 | RM4-5 | ||
| naïve | CD44-CD62L+ | IM7 ; Mel-14 | ||
| effector memory | CD44+CD62L- | IM7 ; Mel-14 | ||
| CD8+ T cell | CD8 | 53-6.7 | ||
| naïve | CD44-CD62L+ | IM7 ; Mel-14 | ||
| effector memory | CD44+CD62L- | IM7 ; Mel-14 | ||
| B cell | B220 | RA-6B2 | ||
| Mature B cells | IGM,CD19 | 11/41 ; ebio1D3 | ||
| Granulocyte | Gr1 | RB6-8C5 | ||
| Macrophage | ||||
| Macrophage | F4/80 | BM8 | ||
| M1 | CD11c | N418 | ||
| M2 | CD206 | C068C2 | ||
| Granulocyte | Gr1 | RB6-8C5 |