Figure 4. Structural context of the isolated mutants.
a) R32V and R32M disrupt a putative salt bridge between two monomers (shown in blue and green) at R32 and E69. The two residues are shown as stick structures colored by atom identity (blue - N; red - O; gray - C). b) 303GVSdup duplicated the loop shown in orange. One of the monomers is shown as a space-filling model. The catalytic site residues (R173, H289, R292, W315, Y324) are shown as stick figures. The crystallographic data was obtained from PDB 3C29.