Table 1. Genome-wide off-target integration frequency.

Insertion frequency corresponds to the ratio of loxP⁺ to loxP⁻ colonies. 95% C.I. were computed using the Poisson variance. The data represent pooled observations from 3–4 independent experiments.

	loxP+ ori- kanR colonies	loxP- ori+ kanR colonies	loxP insertion frequency	95% C.I.
Y324F	1	6.2 × 106	1.6 × 10−7	0 – 4.8 × 10−7
WT	1,025	8.1 × 106	1.3 × 10−4	1.2 × 10−4 – 1.4 × 10−4
R32V	2	9.4 × 106	2.1 × 10−7	0 – 5.1 × 10−7
R32M	3	6.3 × 107	4.8 × 10−8	0 – 1 × 10−7
303GVSdup	46	2.6 × 107	1.7 × 10−6	1.2 × 10−6 – 2.2 × 10−6