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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Open Circ Vasc J. 2011 May 9;4:12–17. doi: 10.2174/1877382601104010012

Fig. (1).

Fig. (1)

Murine microvascular segments suspended in 3D collagen gels. A) A freshly-isolated microvascular segment (arrow). B) A similar segment has produced long sprouts (three are labeled with arrows) from its main body (MB) after 14 days of culture. C) A segment labeled with antibodies to PECAM (red) to identify ECs of the endothelial lining, antibodies to alpha smooth muscle actin (green) to reveal supportive mural cells, and DAPI (blue) to show the cell nuclei. Scale bars in A and C = 100 μm. Scale bar in B = 200 μm.