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Overview of standard iodine staining procedures. The three protocols (I2KI, ethanol I2KI, and I2KI washed with EtOH) on the left were used in this study. Each step positioned at the same horizontal level was performed for the same duration. Note that the I2E/I2M protocol can use either ethanol (EtOH) or methanol (MeOH). All steps that used an iodine solution are highlighted with a gray background for clarity.
