Figure 5.

MicroCT images of quail embryos stained with 3.75% I₂KI and ethanol I₂KI at various stain durations and a Mallory Trichrome histological section for comparison. All CT images are unmodified images obtained from tomographic reconstruction software and at the same scale. An embryo stained with I₂KI for 14 days and subsequently washed with 75% ethanol is presented in the right side of an embryo stained with I₂KI for 14 days for direct comparison. All the microCT images are from approximately the same cross-sectional level illustrated in the top left. The histological image is magnified 1.8 times to be comparable to a size of all microCT images. The extreme shrinkage of the histological sample is an artifact of dehydration and paraffin processing.