Table 1.

Discrimination indices (DI) for the selected organs for unstained embryos and embryos stained with I₂KI, I₂KI washed with ethanol and ethanol I₂KI staining protocols. DI values for I₂KI and ethanol I₂KI for 2 and 7 days staining are excluded in this table because all the selected organs are not saturated with each staining and highly dependent on their depths within the embryos (Fig. 4)

Stain	Time (days)	Discrimination Index
		b – m.s	b – e	b – m.l	b – s	m.s – e	m.s – m.l	m.s – s	e – m.l	e – s	m.l – s	Mean DI
Unstained	0	0.24	0.24	0.25	0.27	0.24	0.24	0.25	0.25	0.27	0.26	0.25
I2KI	14	4.56	1.63	4.21	2.68	1.92	1.28	1.19	2.50	1.39	1.94	2.33
I2KI washed with 75%EtOH	14	9.13	2.51	3.87	2.63	2.62	1.10	3.13	1.96	0.74	1.83	2.95
Ethanol I2KI	14	12.08	1.98	4.32	3.36	1.42	1.12	1.28	1.72	1.66	1.57	3.05
I2KI	28	6.39	1.17	3.73	4.54	3.18	1.17	0.94	2.63	2.64	1.39	2.78
Ethanol I2KI	28	9.54	2.24	4.42	2.36	3.71	1.12	5.34	2.41	0.89	2.02	3.41

b, bronchus; e, esophagus; m.l, musculus longus colli ventralis; m.s, musculus supracoracoideus; s, spinal cord.