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. 2014 Apr 1;9(4):e93413. doi: 10.1371/journal.pone.0093413

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© 2014 Johansson et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cells from two separate isolations were seeded on collagen-coated glass chamber slides and fixed on days 1, 2, and 10. PTRF/CAVIN-1 (red) and caveolin-1 (green) were detected by immunofluorescence; DAPI (nuclei, blue) was supplied in the mounting medium. Nuclear PTRF (day 1) becomes cytoplasmic and co-localizes with caveolin-1 by day 10. Results shown are representative. Original magnification 40X. (B) Paraffin-embedded normal human lung tissue sections were deparaffinized and immunofluorescence was performed for PTRF/CAVIN-1 (red) and caveolin-1 (green, left panel) or SP-C (green, right panel); DAPI (nuclei, blue) was supplied in the mounting medium. Caveolin and PTRF co-localize while PTRF and SP-C do not. Results shown are representative of three separate cellular preparations. Original magnification 100X.