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. 2014 Apr 1;9(4):e93547. doi: 10.1371/journal.pone.0093547

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A,B) Western blot analysis showing the dose- (A) and time- (B) dependent effect of CuB on VASP phosphorylation. (C) Immunofluorescence microscopy analysis showing the co-localization (yellow) of VASP (red) and actin (green) with nuclei (blue) counter staining after 0.1 μM CuB treatment. (D) Co-localization analysis of VASP and actin of C. Both PDM images and ICQ values (right panel) are shown. (E) CuB induced cofilin-actin rod formation. Cells were immunostained with anti-β-actin (green) and anti-cofilin (red) antibodies with nuclei (blue) counter staining. (F) Influence of cofilin knockdown on CuB-induced VASP activation. (G) Influence of cofilin knockdown on actin distribution. Magnified images of the boxed areas (merged images) are presented in C, E and G. Scale bars: 10 μm (5 μm in magnified images).