Table 1. MSD analysis of cytokine (pg/ml) release from hESC-EC.
Analyte (pg/ml) | GM-CSF | IFNγ | IL-10 | IL-12p70 | IL-1β | IL-2 | IL-6 | TNFα | CXCL8 |
CONTROL | 30.3±2.5 | ND | 1.6±0.4 | 8.0±3.6 | 0.8±0.3 | 65.9±5.9 | 90.9±14.5 | ND | 1566.5±160.0 |
+ C12-iE-DAP | 160.9±45.3* | 11.1±7.1 | 6.0±1.7 | 83.7±30.3* | 11.9±1.1* | 303.9±64.8* | 295.5±78.1* | 4.8±0.2* | 9569.1±2645.2* |
+ LPS | 20.9±0.5 | ND | 1.9±0.3 | 12.9±3.2 | 2.1±1.2 | 62.6±4.0 | 104.8±9.1 | ND | 1691.8±120.3 |
Data mean are ± SEM for n = 3. hESC-EC were treated for 24 hours with vehicle, LPS (1 µg/ml), or C12-iE-DAP (10 µg/ml). Statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparison test (*p<0.05). ND = non-detectable.