Table 2. MSD analysis of cytokine (pg/ml) release from HUVEC.
Analyte (pg/ml) | GM-CSF | IFNγ | IL-10 | IL-12p70 | IL-1β | IL-2 | IL-6 | TNFα | CXCL8 |
CONTROL | 37.0±1.6 | 2.8±1.0 | 1.4±1.2 | 12.9±0.9 | 1.3±0.6 | 69.3±19.2 | 63.7±20.4 | 0.5±0.3 | 1500.6±351.5 |
+ C12-iE-DAP | 190.0±44.8 | 7.9±3.0 | 2.7±1.1 | 60.2±12.5* | 7.0±3.3 | 198.2±26.3* | 213.1±65.1 | 1.7±0.9 | 6044.1±960.3* |
+ LPS | 279.±115.5 | 5.1±1.3 | 4.7±0.6 | 38.7±5.0 | 5.6±2.4 | 223.1±34.3* | 323.8±114.2 | 1.8±1.0 | 6154.4±857.6* |
Data are mean ± SEM for n = 3. HUVEC were treated for 24 hours with vehicle, LPS (1 µg/ml), or C12-iE-DAP (10 µg/ml). Statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparison test (*p<0.05).