Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 1;9(4):e93598. doi: 10.1371/journal.pone.0093598

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 McIntosh et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

M1 was coupled to HBcAg (residues 1–142) using the heterobifunctional cross-linker sMBS (Pierce). Western Blot indicating the coupled product is recognised by both the anti-M1 and anti-HBcAg antibodies. (B) Following conjugation, the M1:HBcAg was purified from unbound M1 protein by ultracentrifugation. The resulting coupled product was analysed by SDS-PAGE under reducing conditions followed by silver stain. Following purification no contaminating M1 was observed in the purified product. HBcAg (C) and M1:HBcAg (D) were stained with uranyl acetate and analysed by electron microscopy. The morphology of HBcAg was retained following coupling and purification.