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. 2014 Apr 1;9(4):e93598. doi: 10.1371/journal.pone.0093598

Figure 4. M1:HBcAg is presented on MHCI and activates HBcAg-specific CD8+ T cells.

Figure 4

Immature DCs were pulsed with M1:HBcAg or the control antigens for 60 minutes at room temperature. Following extensive washing, the cells were incubated overnight at 37°C. Antigen-loaded cells or untreated cells (UT) were incubated with HBc18–27 cells for 6 hours in the presence of Brefeldin A and monensin. CD107a (A&C) expression and IFNγ (B&D) production were measured in parallel. Results in A and B indicate the T cell activation as a percentage of the positive control of DCs loaded with the molar equivalent of C18–27 peptide. Data are representative of 4 individual experiments. M1:HBcAg treatment resulted in significantly more T cell clones expressing CD107a and IFNγ than the control antigens. *Denotes statistical significance determined by student t-test, <0.05. A representative data set from one individual is presented in C & D showing mean and standard deviation of duplicate samples.