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. 2014 Apr 1;9(4):e92408. doi: 10.1371/journal.pone.0092408

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© 2014 Vonk et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. HEK293T cells were co-transfected with SOD1-Flag mutants (A4V, G37R, G85R, D90A, G93A, E100G) in combination with EV (–) or HA-COMMD1 prior to lysis and detection of SOD1 multimeric complexes using SDS-PAGE under non-reducing conditions (– β-ME; 40 μg). Immunoblotting was performed using antibodies directed against the Flag-tag and HA-COMMD1 proteins. Input samples were analysed in presence of β-ME (+ β-ME; 30 μg). Samples were analysed on separated membranes. B. HEK293T cells were co-transfected with SOD1-Flag WT in combination with EV (–) or HA-COMMD1 prior to lysis and analysed as described in Figure 3A. C. Visualization of SOD1 HMW complexes in Neuro2A cells transfected with SOD1-Flag mutants G85R and G93A in combination with EV (–) or HA-COMMD1 as described in Figure 3A. Samples were analysed on separated membranes.