Skip to main content
. 2014 Apr 1;9(4):e92408. doi: 10.1371/journal.pone.0092408

Figure 4. COMMD1 is in complex with aggregated mSOD1 proteins.

Figure 4

A. Neuro2A cells were transient transfected with eYFP-tagged SOD1 WT, or mSOD1 G85R and G93A, with or without mCherry-COMMD1, as indicated. 72 h post-transfection, cells were fixed and imaged using a confocal microscope. B. HEK293T cells were transient transfected with either EV (–) or HA-COMMD1 in combination with EV, or Flag-tagged mSOD1 G85R or G93A constructs prior to lysis and detection of SOD1 multimeric complexes using blue native PAGE (30 μg). Immunoblotting was performed using antibodies directed against the Flag-tag and HA-COMMD1 proteins. Total protein levels were analysed in presence of β-ME on a SDS-PAGE gel (30 μg). Photoshop analysis was used to generate overlay, using a marked region on the membrane as focus point.