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. 2014 Apr 1;9(4):e92408. doi: 10.1371/journal.pone.0092408

Figure 5. COMMD1-mediated induction of mSOD1 aggregation is affected by a SOD1 C6S substitution.

Figure 5

A. HEK293T cells were transient transfected with either EV (–) or HA-COMMD1 in combination with GST only or SOD1-GST constructs (WT, C6S, C111S). GST PD was performed as described under Figure 1A. B. HEK293T cells were transient transfected with EV (–) or HA-COMMD1 in combination with GST only or SOD1-GST constructs (WT, C6S, G93A, C6S/G93A, G85R, C6S/G85R). GST PD was performed as described under Figure 1A. C. Filter trap analysis of HEK293T cells transiently transfected with SOD1-Flag mutants (C6S, G93A, C6S/G93A) in combination with either EV (–) or HA-COMMD1. Detection of aggregates was performed as described in Figure 2A.