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. 2014 Apr 1;9(4):e93273. doi: 10.1371/journal.pone.0093273

Figure 5. Effect of 5-azaC on DNA double strand break repair in NPC cells in vitro.

Figure 5

CNE2 and SUNE1 cells were pretreated with 5-azaC, irradiated at 6Gy and subjected to γH2AX staining at the indicated times. (A) Images of γH2AX-positive foci (pink) and DAPI nuclear counterstaining (blue). (B) Double strand break repair kinetics (Student’s t-test, p>0.05). (C) Western blot assays of the expression levels of DNA-PK, Phospho-DNA-PK (Ser2056), XRCC4, XLF, RPA2 and RAD51 over time in CNE2 and SUNE1 cells after IR.