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. 2014 Apr 1;9(4):e93273. doi: 10.1371/journal.pone.0093273

Figure 6. Effect of 5-azaC on the DNA methylation and expression of representative tumor suppressor genes that are hypermethylated and silenced in NPC.

Figure 6

SUNE1 and CNE2 cells and mice bearing CNE2 tumor xenografts were treated with or without 5-azaC. (A) DNA methylation pyrograms for RASSF1A and RPRM in CNE2 cells. (B) Mean levels of DNA methylation for RASSF1A, RPRM, CDKN2A and 14-3-3σ (Student’s t-test, *p<0.05, **p<0.01). (C) Real-time PCR analyses of RASSF1A, RPRM, CDKN2A and 14-3-3σ (Student’s t-test, **P<0.01).