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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2014 Feb 28;133(4):1203–1205.e7. doi: 10.1016/j.jaci.2013.12.1086

Figure 1.

Figure 1

(A) Lymphocytes were identified from whole PBMCs (left) and lineage-negative cells gated (middle). Lineage-negative lymphocytes were further assessed for expression of CD127 and CRTH2 or isotype control staining (right). ILC2 were identified as lineage-negative CRTH2+ lymphocytes. Example shown is 4 hours after cat allergen exposure in cat allergic individual (B) ILC2 (left) and CRTH2+ CD4+ cells (middle) were analyzed for baseline CD84 expression in cat allergic individuals and change in MFI compared with respective isotype determined (right). **P < 0.005, t-test, n= 5 individual subjects.