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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Eur J Nutr. 2013 Oct 4;53(3):843–852. doi: 10.1007/s00394-013-0588-5

Fig. 2.

Fig. 2

SFN treatment causes induction of vimentin protein in a normal prostate epithelial cell line (PrEC) but not in a normal prostate stromal cell line (PrSC). a Western blotting for snail, slug, and Zeb1 proteins using lysates from PC-3 and DU145 cells after treatment with DMSO (control) or the indicated concentrations of SFN for specified time periods. b Western blotting for vimentin and E-cadherin using lysates from PrEC and PrSC cells (E-cadherin protein was not detectable in the PrSC cell line) after treatment with DMSO (control) or the indicated concentrations of SFN for specified time periods. Blots were stripped and reprobed with anti-actin antibody. Numbers on top of bands are fold changes relative to corresponding DMSO-treated control. Each experiment was repeated at least twice. c Densitometric quantitation of vimentin and E-cadherin protein expression changes (arbitrary units) from western blots shown in panel b. Results shown are mean ± SD (n=2). Quantitation was normalized against actin protein band intensity for each individual experiment.