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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Eur J Nutr. 2013 Oct 4;53(3):843–852. doi: 10.1007/s00394-013-0588-5

Fig. 4.

Fig. 4

Effect of vimentin knockdown on SFN-induced apoptosis. a Western blotting for vimentin protein using lysates from PC-3 cells transiently transfected with a control siRNA or a vimentin-targeted siRNA. Flow cytometric analysis of b early (Annexin V+) or c late apoptosis (Annexin V+ and propidium iodide+) in PC-3 cells transiently transfected with a control siRNA or a vimentin-targeted siRNA and treated for 24 h with DMSO or SFN. Results shown are mean ± SD (n=3). aSignificantly different (P < 0.05) compared with corresponding DMSO-treated control by one-way ANOVA followed by Bonferroni’s multiple comparison test. Difference was not significant between control siRNA and vimentin siRNA transfected cells at any dose of SFN. Each experiment was repeated at least twice, and representative data from one such experiment are shown.