Table 1.
Human Forward and Reverse Primers for Real Time polymerase chain reaction
| Gene | Primer sequence | Amplicon length | NCBI Accession# |
|---|---|---|---|
| IL-1β Forward | 5-CAA ATT CGG TAC ATC CTC GAC -3 | 73 | NM_000576 |
| IL-1β Reverse | 5-GTC AGG GGT GGT TAT TGC ATC -3 | ||
| IL6 Forward | 5-AAA CAG ATG AAG TGC TCC TTC CAG G -3 | 339 | NM_000600 |
| IL6 Reverse | 5-TGG AGA ACA CCA CTT GTT GCT CCA-3 | ||
| IL13 Forward | 5-TGA GGA GCT GGT CAA CAT CA-3 | 76 | NM_002188 |
| IL13 Reverse | 5-CAG GTT GAT GCT CCA TAC CAT-3 | ||
| TNFα Forward | 5-CAG AGG GAA GAG TTC CCC AG -3 | 84 | NM_000594 |
| TNFα Reverse | 5-CCT TGG TCT GGT AGG AGA CG-3 | ||
| Connexin-43 Forward | 5′-CCT ATG TCT CCT CCT GGG TA-3′ | 176 | NM_000615 |
| Connexin-43 Reverse | 5′-GGG AAA TCA AAA GGC TGT G-3′ | ||
| Prostaglandin F2α Receptor Forward | 5-GCA GCT GCG CTT CTT TCAA-3 | 81 | NM_000959 |
| Prostaglandin F2α Receptor Reverse | 5-CAC TGT CAT GAA GAT TAC TGA AAA AAA TAC-3 | ||
| Oxytocin Receptor Forward | 5-CTG AAC ATC CCG AGG AAC TG-3 | 84 | NM_000916 |
| Oxytocin Receptor Reverse | 5-CTC TGA GCC ACT GCA AAT GA-3 | ||
| GAPDH Forward | 5-TGA TGA CAT CAA GAA GGT GGT-3 | 240 | NM_002046 |
| GAPDH Reverse | 5-TCC TTG GAG GCC ATG TGG GCC-3 |