Fig. 5.

MSK is the CREB kinase responsible for AngII-mediated signals. Panel a Quiescent VSMCs were treated with 1 μM AngII for 10 min. Kinase phosphorylation was assessed by Western blotting of 5 μg of total starting protein. Panel b Quiescent VSMCs were pre-treated for 15 min with the indicated inhibitors (CID: 10 μM; BI: 10 μM & SB: 5 μM) and subsequently treated for 10 min with 1 μM AngII. CREB phosphorylation was assessed by Western blotting of 5 μg of total starting protein. Panel c Quiescent VSMCs were pre-treated with Bisindol (10 μM) and subsequently treated for 10 min with 1 μM AngII. CREB and MSK phosphorylation was assessed by Western blotting of 5 μg of total starting protein. All results are plotted as means ± SEM (n = 3); *Significantly different from untreated controls by one-way ANOVA, (p < 0.05)