Fig. 1.

Human non-pigmented ciliary epithelial cells express MAGP-1 mRNA and protein. Human non-pigmented ciliary epithelial cells were cultured for 7 days, and cDNA was amplified with specific primers for MAGP-1, MAGP-2, and the housekeeping gene GAPDH. A MAGP-1 band corresponding to 498 bp was clearly detected, whereas the MAGP-2 band corresponding to 526 bp was fainter than the MAGP-1 band (A). Western blot of cell/matrix lysates (5 µg) of non-pigmented ciliary epithelial cells cultured for 7 days (B). The blots were obtained using anti-MAGP-1 and MAGP-2 antibodies. The two probes recognized bands of 21 and 19 kDa, respectively. MAGP-1 was detected at an estimated 21 kDa, whereas MAGP-2 was not detected (not shown).