Fig. 5.

HMGB1 siRNA and RAGE siRNA attenuated invasion and mobility of HCCLM3 cells in vitro. HCCLM3 cells were seeded into the upper chamber of the transwell, treated with HMGB1-siRNA, RAGE-siRNA, anti-RAGE antibody or sRAGE, and rhHMGB1, and allowed to invade matrigel for 24 h. a The invasive cells migrating through the basal membrane to its lower surface were stained with crystal violet, then were photographed (20 × 10). b The number of invasive cells was also quantified by dissolving the purple crystals on the membranes in 500 μl 10 % acetic acid, and measuring their OD values at 570 nm by Multiskan Ascent. Cell invasion ability was expressed indirectly by varying OD values. HMGB1 or RAGE siRNA, HMGB1, or RAGE antibody, and sRAGE inhibited the invasion ability of HCCLM3 cells, while rhHMGB1 facilitated it (*P < 0.05, **P < 0.01). c, d Migration ability of HCCLM3 cells was detected by wound healing assay. Incubating for 0, 6, 12, and 24 h, respectively, the number of HCCLM3 cells migrating into the scraped areas was counted. (*P < 0.05, **P < 0.01)