Fig. 6.

Effects of HMGB1 and RAGE on NF-κB expression in HCCLM3 cells. a Expression of NF-κB p65 or p50 mRNA in HCCLM3 cells was explored by RT-PCR. b Relative expression of NF-κB p65 or p50 mRNA was normalized to β-actin. HMGB1 or RAGE siRNA, HMGB1 or RAGE antibody, and sRAGE inhibited NF-κB p65 and p50 mRNA expression in HCCLM3 cells, while rhHMGB1 increased it (* P < 0.05, ** P < 0.01). c Western blot was performed to test NF-κB p65 or p50 protein expression in HCCLM3 cells. d Quantity analysis of NF-κB p65 and p50 proteins expression levels relative to GAPDH. (* P < 0.05, ** P < 0.01)