Skip to main content
. 2014 Apr 2;4:4569. doi: 10.1038/srep04569

Figure 2. Loss- or gain-of-function analysis of hNav1.5 and hNav1.7 chimeras.

Figure 2

The wild type (WT) or chimeric VGSC currents were recorded before (black trace) and after (red trace) the application of JZTX-III. Panel A, inhibition of 1 μM JZTX-III on WT-hNav1.5 and chimeric hNav1.5 currents with S3–S4 fragments replaced by those from hNav1.7. M1.5-1, M1.5-2, M1.5-3, and M1.5-4 represent chimeras with the S3–S4 fragments of DII, DIII, DIV, and all three domains (DII + DIII + DIV) of hNav1.5 replaced by those from hNav1.7, respectively. Panel B, inhibition of 1 μM JZTX-III on hNav1.7 currents, the naming of the chimeras followed the same rules as for the hNav1.5 chimeras. Panel C, quantitation of the inhibition percentage of JZTX-III (1 μM) on chimeras' currents. *, p < 0.05; **, p < 0.001 significance between WT and chimeric channels. Panel D, concentration-response inhibitory curves of JZTX-III. Data points (mean ± S.E., each from 3–5 cells).