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. 2014 Apr 2;4:4570. doi: 10.1038/srep04570

Figure 7. ZBED6 and IGF2 expression change after ZBED6 knockdown in bovine primary myocyte cells.

Figure 7

(A) Comparison of mRNA expression levels of bovine ZBED6 and IGF2 between transfected with Ad-sh906 and Ad-sh2819 (transduced with two adenoviruses at 300 multiplicity of infection for 48 hrs) in decreasing ZBED6 expression in bovine primary myocyte cells. A blank expression vector (Ad-NC) was used to maintain equivalent amounts of DNA. The mRNA expression was normalized to the geometric mean of the suitable housekeeping genes (GAPDH and ACTINB) and expressed relative to gene expression in the control group. Error bars represent standard error of the mean (SEM). Each column value represents the mean ± SEM of at least three independent experiments (n = 3 or 4). *P < 0.05. (B) Semi-qCR was performed to determine the endogenous expression level of ZBED6 and IGF2 using mRNA isolated from bovine primary myocyte cells. Data are representative of at least three independent experiments performed in duplicate (n = 3 or 4). (C) Crude proteins were extracted from the treated cells and then subjected to Western blot with antibodies against ZBED6 and IGF2, GAPDH was used as a control for equal protein loading. Data are representative of three independent experiments performed in duplicate (n = 3). The data revealed that Ad-sh1906 had the highest knockdown of ZBED6 transcript and protein.