Table 1.
Primers and probes used in this study
| Marker and cytokine gene | Sequence (5′-3′) | Product Size (pb) | ID |
|---|---|---|---|
| B-actin |
F: CGAGCGCGGCTACAGCTT |
58 pb |
NM_001101 |
| R: CCTTAATGTCACGCACGATT | |||
| P: ACCACCACGGCCGAGCGG | |||
| FasL |
F: CTGGGGATGTTTCAGCTCTTC |
231 |
NM_152872 |
| R: GTCCTGCTTTCTGGAGTGAAG | |||
| Fas |
F: AAGGAGTACACAGACAAAGCCC |
170 |
NM_000043.3 |
| R: GGGTGGCTTTGTCTTCTTCTT | |||
| COX1 |
F:AGCAGCTTTTCCAGACGACC |
157 pb |
NM_000962 |
| R:CGGTTGCGGTATTGGAACTG | |||
| P: CTGGCCTCAGCACTCTGGAATGACAA | |||
| COX2 |
F: CCTGATCCCCAGGGCTCAAAC |
79 pb |
NM_000963 |
| R: TTGGTGAAAGCTGGCCCTCG | |||
| P: TGCCCAGCACTTCACGCATCAGTT | |||
| VEGF |
F: TCTACCTCCACCATGCCAAGT |
102 pb |
AB021221 |
| R: TGCGCTGATAGACATCCATGA | |||
| P: CCAGGCTGCACCCATGGCAGA | |||
| HIF |
F: CCAAATCCAGAGTCACTGGAACTT |
96 pb |
NM_001530 |
| R: AGGTGAACTTTGTCTAGTGCTTCCAT | |||
| P: TACCATGCCCCAGATTCAGGATCAGACAC | |||
| IL-2 |
F: TGATTTTGAATGGAATTAATAATTACAAG |
95 pb |
NM_000586.3 |
| R: TTTCAGTTCTGTGGCCTTCTT | |||
| P: CCCAAACTCACCAGGATGCTCACATT | |||
| IL-4 |
F: GCCTCACAGAGCAGAAGACTC |
75 pb |
NM_172348.1 |
| R: CAGTTGTGTTCTTGGAGGCA | |||
| P: TGCACCGAGTTGACCGTAACAGACA | |||
| IL-10 |
F: CCTGGAGGAGGTGATGCCCCA |
131 pb |
NM_000572.2 |
| R: CAGCGCCGTAGCCTCAGCC | |||
| P: CAAGGCGCATGTGAACTCCCTG | |||
| IFNγ |
F: GAAGAATTGGAAAGAGGAGAGTGA |
218 pb |
NM_029419.12 |
| R: TGGACATTCAAGTCAGTTACCG | |||
| P: TTCCTTGATGGTCTCCACACTCTTTTGG | |||
| TNF | F. GCCCAGGCAGTCAGATCA |
74 pb | NM_000594.2 |
| R: GCTTGAGGGTTTGCTACAACA | |||
| P: CCCGAGTGACAAGCCTGTAGCCC |