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. 2013 Jan 4;2(1):19–42. doi: 10.3390/cells2010019

Figure 1.

Figure 1

Identification of epitopes in ICP27, VP22 and VP13/14 from HSV-2 infected BALB/c mice. BALB/c mice were infected with 1 × 103 pfu of HSV-2 (strain 333) through intra-vaginal administration. After 14 days splenocytes were obtained and pulsed individually (5 × 105 cells) with 10 µg/mL of (A) ICP27, (B) VP22 and (C) VP13/14 derived peptides in an overnight IFN-γ ELISPpot assay. Counts are presented as spot forming cells per million splenocytes with standard error of means (SEM). Cut off values 2 SD over background were considered significant and spots reaching 90 SFU or above were defined as positive.