Skip to main content
. 2013 Jan 4;2(1):19–42. doi: 10.3390/cells2010019

Figure 4.

Figure 4

Mobilization of CD107a on peptide specific CD8+ T cells after DNA vaccine administration encoding the HSV-2 ICP27, VP22 and VP13/14 antigens. (A) BALB/c mice (n = 6) were immunized using the DNA prime-boost regimen using particle mediated epidermal delivery with full length monovalent vaccines (ICP27, VP22 and VP13/14). On day 40 splenocytes were harvested, pooled in their respective groups and pulsed with peptide for 4 hours in the presence of anti-CD107a-FITC antibody. (B) FACS plots are shown as CD107a+/CD8+ T cells as a percentage of total CD8+ T cells for VP22, VP13/14 and ICP27 peptide specific response. Irrelevant peptide control and ConA CD107a+/CD8+ responses are shown as negative and positive controls for CD107a degranulation. (C, D, E, F) Percentage of CD107a+/CD8+ T cell responses shown with irrelevant peptide responses subtracted from the total CD107a+/CD8+ T cell percentages for each of the HSV-2 DNA immunization group. (E) Graph showing CD107a+/CD8+ T cell responses in mice immunized with a mock antigen and pulsed with ICP27, VP22 and VP13/14 peptides. (G) Graph showing CD107a+, CD8+ T cell degranulation after ConA stimulation.