Table 1.
Sequence information and predicted IC50 (nM) binding values for ICP27, VP22 and VP13/14 selected peptides.
| Epitope | Peptide Sequence | Predicted a IC50 (nM) for Kd | Predicted a IC50 (nM) for Dd | Predicted a IC50 (nM) for Ld | Predicted a IC50 (nM) for HLA-A2 |
|---|---|---|---|---|---|
| ICP27(314-322) | TLVAHGPSL | 29704.2 | 29811.3 | 29903.8 | 172.1 |
| ICP27(318-326) * | HGPSLYRTF * | 29523.2 | 41.4 | 26435.4 | 24491.7 |
| ICP27(319-327) | GPSLYRTFA | 37218.7 | 32191.1 | 36928.3 | 24083.7 |
| ICP27(424-432) | DIASFVLVI | 32248.6 | 16323.4 | 44299.6 | 12567.5 |
| ICP27(425-433) | IASFVLVIL | 27247.8 | 29428.1 | 38689.5 | 5961 |
| ICP27(427-435) | SFVLVILAR | 31071.1 | 33613.3 | 45487.2 | 23983 |
| ICP27(428-436) | FVLVILARL | 15291.9 | 31066.4 | 39404.5 | 44.2 |
| ICP27(459-467) | TMHFYIPGA | 39602.4 | 29117.4 | 44287.4 | 37.3 |
| ICP27(462-470) | FYIPGACMA | 2073.3 | 30525.3 | 43726.8 | 13482.9 |
| ICP27(464-472) | IPGACMAGL | 22701.7 | 8136.7 | 322.7 | 21245.3 |
| ICP27(479-487) | HRQECSSRV | 32214.5 | 31069.2 | 39806.7 | 19930.4 |
| ICP27(482-490) | ECSSRVCEL | 31674.4 | 23031 | 43090.6 | 22306.7 |
| ICP27(484-492) | SSRVCELTA | 37431.5 | 29921.3 | 44401.6 | 23541.2 |
| ICP27(499-507) | LYVHGKYFY | 31170.9 | 31676 | 42144.7 | 24253.4 |
| ICP27(500-508) | YVHGKYFYC | 39488.8 | 31902.1 | 31410.8 | 4141.3 |
| ICP27(502-510) | HGKYFYCNS | 39256 | 30676.7 | 44620 | 24494 |
| ICP27(504-512) | KYFYCNSLF | 1731.1 | 19533.6 | 35494.7 | 22076.5 |
| VP22(46-54) | MRARPRGEV | 33458 | 31986.6 | 40180.1 | 22734.7 |
| VP22(49-57) | RPRGEVRFL | 35468.7 | 32591.2 | 15593.8 | 24194.2 |
| VP22(144-152) | PAQADSAVL | 31273.4 | 27008 | 39887.1 | 22567.6 |
| VP22(146-154) | AQADSAVLL | 28650.2 | 29610.5 | 39773.3 | 219.8 |
| VP22(170-178) | QGLAKKLHF | 36005.8 | 25105.8 | 28660.9 | 24422.6 |
| VP22(171-179) | GLAKKLHFS | 38907.3 | 30342.7 | 44645.9 | 3066.5 |
| VP22(172-180) | LAKKLHFST | 36466.3 | 32481.7 | 41828.9 | 22976.2 |
| VP22(173-181) | AKKLHFSTA | 36250.5 | 33000.5 | 44582.4 | 24606.2 |
| VP22(197-205) | NKRVFCAAV | 30008.2 | 31775.2 | 44919.6 | 22655.2 |
| VP22(200-208) | VFCAAVGRL | 1306.3 | 32999.6 | 9018.9 | 21578.2 |
| VP13/14(224-232) | FYPCPDSAF | 16119.3 | 1274.8 | 39803 | 24361.5 |
| VP13/14(225-233) | YPCPDSAFG | 39192.9 | 34274.1 | 34907.2 | 24514.4 |
| VP13/14(228-236) | PDSAFGLSR | 39770.5 | 33330.2 | 45411.5 | 24833.9 |
| VP13/14(229-237) | DSAFGLSRV | 31063.7 | 29721.2 | 44388.9 | 21332.2 |
| VP13/14(242-250) | FASPADPKV | 36540 | 26313.6 | 7025.5 | 743 |
| VP13/14(243-251) | ASPADPKVF | 37288.4 | 6838.7 | 31207.7 | 24557.9 |
| VP13/14(244-252) | SPADPKVFF | 37123.2 | 13334.2 | 232.3 | 24985.9 |
| VP13/14(394-402) | TYIATGALL | 21.5 | 25985.1 | 34758.1 | 19830.3 |
| VP13/14(395-403) | YIATGALLA | 37750 | 31700.8 | 44153.7 | 162.8 |
| VP13/14(414-422) | LPREAAFAG | 39383.4 | 33334.8 | 26281.3 | 24771.1 |
| VP13/14(415-423) | PREAAFAGR | 39228.2 | 32630.7 | 43207.1 | 24858.4 |
| VP13/14(419-427) | AFAGRVLDV | 25977.5 | 20910.7 | 40665.1 | 11777.1 |
| VP13/14(421-429) | AGRMTYIAT | 36221.7 | 30484.7 | 44486.5 | 24623 |
| VP13/14(589-597) | TQPLYARTT | 39452.7 | 25084.5 | 45112 | 22116.8 |
| VP13/14(590-598) | QPLYARTTP | 28489.2 | 31596.9 | 30047.2 | 24585.7 |
| VP13/14(591-599) | PLYARTTPA | 28610.6 | 28076.8 | 44528.9 | 2426.4 |
| VP13/14(592-600) | LYARTTPAK | 23103.7 | 30223.7 | 45027.4 | 24767.2 |
* Denotes a previously described epitope in BALB/c mice (see reference [18]); a Predictions were performed using IEDB www.immuneepitope.org (see reference [24] and methods); b Known HLA-A2 binder.