Table 2.
Summary of epitopes identified from HSV-2 proteins in BALB/c mice after infection and immunization with ICP27, VP22 and VP13/14 DNA vaccines.
| BALB/c Epitope | Peptide Sequence | IFN-γ Responses (SFC) after HSV-2 Infection in BALB/c mice (n = 4) | IFN-γ Responses (SFC) in Mice Immunized with Full Length DNA Vaccine (n = 6) ψ | IFN-γ Responses (SFC) in Uninfected BALB/c Mice (n = 2) |
|---|---|---|---|---|
| ICP27(318-326) * | HGPSLYRTF | 436.2 | 304.4 | 16.5 |
| ICP27(428-436) | FVLVILARL | 129.7 | 16.9 | 7.5 |
| ICP27(459-467) | TMHFYIPGA | 288.5 | 71 | 5.5 |
| ICP27(479-487) | HRQECSSRV | 98.75 | 12.5 | 5.5 |
| ICP27(500-508) | YVHGKYFYC | 357.7 | 247 | 4.5 |
| VP22(46-54) | MRARPRGEV | 354.7 | 4.5 | 3 |
| VP22(171-179) | GLAKKLHFS | 311.2 | 23.8 | 8 |
| VP22(200-208) | VFCAAVGRL | 109.2 | 189.6 | 9.5 |
| VP13/14(244-252) | SPADPKVFF | 330.7 | ND ** | 4.5 |
| VP13/14(592-600) | LYARTTPAK | 330 | ND ** | 8.5 |
* Denotes a previously described epitope in BALB/c mice (see reference [18]); ψ Mice immunized with DNA vaccine encoding ICP27, VP22 or VP13/14 using PMED device. Naïve mice immunized with DNA encoding mock antigen showed mean background responses for individual peptides less than 5 SFU/million splenocytes (n = 6)’ ** ND (Not determined).